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has3  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology has3
Hyaluronan content and HAS expression in human ovary and its tumors . A) Strong hyaluronan staining intensity (%) in NO (n = 4), SCA (n = 10), BL (n = 4), G1–2 (n = 10) and G3 (n = 10) tissues. Relative mRNA levels of B) HAS2 and C) <t>HAS3</t> in NO (n = 5), SCA (n = 10), BL (n = 4), G1+2 (n = 10) and G3 (n = 10) tissues. The boxes show the ranges between 25th and 75th percentiles, with a horizontal line at the median value. The whiskers extend to the 10th and 90th percentiles. The open circles represent the statistical outlier values. NO = normal ovary, SCA = serous cystadenoma, BL = serous borderline tumor, G1–2 = grade 1–2 serous cystadenocarcinoma, G3 = grade 3 serous cystadenocarcinoma.
Has3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hyaluronan content and HAS expression in human ovary and its tumors . A) Strong hyaluronan staining intensity (%) in NO (n = 4), SCA (n = 10), BL (n = 4), G1–2 (n = 10) and G3 (n = 10) tissues. Relative mRNA levels of B) HAS2 and C) HAS3 in NO (n = 5), SCA (n = 10), BL (n = 4), G1+2 (n = 10) and G3 (n = 10) tissues. The boxes show the ranges between 25th and 75th percentiles, with a horizontal line at the median value. The whiskers extend to the 10th and 90th percentiles. The open circles represent the statistical outlier values. NO = normal ovary, SCA = serous cystadenoma, BL = serous borderline tumor, G1–2 = grade 1–2 serous cystadenocarcinoma, G3 = grade 3 serous cystadenocarcinoma.

Journal: BMC Cancer

Article Title: Expression of Hyaluronan Synthases ( HAS1–3 ) and Hyaluronidases ( HYAL1–2 ) in Serous Ovarian Carcinomas: Inverse Correlation between HYAL1 and Hyaluronan Content

doi: 10.1186/1471-2407-9-143

Figure Lengend Snippet: Hyaluronan content and HAS expression in human ovary and its tumors . A) Strong hyaluronan staining intensity (%) in NO (n = 4), SCA (n = 10), BL (n = 4), G1–2 (n = 10) and G3 (n = 10) tissues. Relative mRNA levels of B) HAS2 and C) HAS3 in NO (n = 5), SCA (n = 10), BL (n = 4), G1+2 (n = 10) and G3 (n = 10) tissues. The boxes show the ranges between 25th and 75th percentiles, with a horizontal line at the median value. The whiskers extend to the 10th and 90th percentiles. The open circles represent the statistical outlier values. NO = normal ovary, SCA = serous cystadenoma, BL = serous borderline tumor, G1–2 = grade 1–2 serous cystadenocarcinoma, G3 = grade 3 serous cystadenocarcinoma.

Article Snippet: Thereafter the sections were incubated overnight at 4°C with polyclonal antibodies for HAS1 (1:100 dilution in 1% BSA, Santa Cruz Biotechnology, inc., Santa Cruz, CA), HAS2 (1:50, Santa Cruz) or HAS3 (1:100, Santa Cruz), followed by a 1 h incubation with biotinylated antigoat antibody (1:1000, Vector Laboratories).

Techniques: Expressing, Staining

Distribution of HAS immunoreactivity in ovarian cancer . Sections of ovarian cancer tissues were stained with antibodies against HAS1, HAS2 and HAS3, as indicated in the panels. The brown color indicates HAS, nuclei are stained with haematoxylin (blue). Note that HAS1 and HAS3 immunoreactivities reside mostly in the nests of the tumor epithelial cells, while HAS2 is present also in the stromal cells. Scale bar 50 μm.

Journal: BMC Cancer

Article Title: Expression of Hyaluronan Synthases ( HAS1–3 ) and Hyaluronidases ( HYAL1–2 ) in Serous Ovarian Carcinomas: Inverse Correlation between HYAL1 and Hyaluronan Content

doi: 10.1186/1471-2407-9-143

Figure Lengend Snippet: Distribution of HAS immunoreactivity in ovarian cancer . Sections of ovarian cancer tissues were stained with antibodies against HAS1, HAS2 and HAS3, as indicated in the panels. The brown color indicates HAS, nuclei are stained with haematoxylin (blue). Note that HAS1 and HAS3 immunoreactivities reside mostly in the nests of the tumor epithelial cells, while HAS2 is present also in the stromal cells. Scale bar 50 μm.

Article Snippet: Thereafter the sections were incubated overnight at 4°C with polyclonal antibodies for HAS1 (1:100 dilution in 1% BSA, Santa Cruz Biotechnology, inc., Santa Cruz, CA), HAS2 (1:50, Santa Cruz) or HAS3 (1:100, Santa Cruz), followed by a 1 h incubation with biotinylated antigoat antibody (1:1000, Vector Laboratories).

Techniques: Staining

Immunostaining of HAS1, HAS3 and HAS2 in human ovary and its tumors

Journal: BMC Cancer

Article Title: Expression of Hyaluronan Synthases ( HAS1–3 ) and Hyaluronidases ( HYAL1–2 ) in Serous Ovarian Carcinomas: Inverse Correlation between HYAL1 and Hyaluronan Content

doi: 10.1186/1471-2407-9-143

Figure Lengend Snippet: Immunostaining of HAS1, HAS3 and HAS2 in human ovary and its tumors

Article Snippet: Thereafter the sections were incubated overnight at 4°C with polyclonal antibodies for HAS1 (1:100 dilution in 1% BSA, Santa Cruz Biotechnology, inc., Santa Cruz, CA), HAS2 (1:50, Santa Cruz) or HAS3 (1:100, Santa Cruz), followed by a 1 h incubation with biotinylated antigoat antibody (1:1000, Vector Laboratories).

Techniques: Immunostaining, Staining